DNAzol Reagent, for isolation of genomic DNA from solid and liquid samples from Thermo Fisher Scientific

Product Specs
ItemDNAzol Reagent, for isolation of genomic DNA from solid and liquid samples
CompanyThermo Fisher Scientific
Price Supplier Page View Company Product Page
Catalog Number10503-027
Brand/TrademarkInvitrogen™
Quantity100 mL
ApplicationsPCR, southern blotting, sequencing, nucleic acid labeling, hybridization
Sample TypeBacteria, Cells, Plant, Tissue, Yeast
Time30 min

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Recombinant cellular model system for human muscle-type nicotinic acetylcholine receptor α12β1δεCell Stress and ChaperonesNovember 25, 2023Sabrina Brockmöller, Thomas Seeger, Franz Worek, Simone Rothmillerat 58×g for 5 min. The supernatant was discarded and the pellet was resuspended and lysed in DNAzol (10503027 Invitrogen) in a ratio of 20 × 106 cells to 1 mL DNAzol. Precipitation of DNA was performed with 100%

(2) CRISPR/Cas9-mediated knock-in in ebony gene using a PCR product donor template in DrosophilaGene and Genome EditingJuly 10, 2023Kathy Clara Bui, Daichi Kamiyamaknock-in. Genomic DNA extraction Genomic DNA was extracted from individual flies using DNAzol (Invitrogen #10503027) or PureLink Genomic DNA Mini Kit (Invitrogen #K182001), according to the manufacturer’s protocol. DNA

(3) Variation in CD8 T cell IFNγ differentiation to strains of Toxoplasma gondii is characterized by small effect QTLs with contribution from ROP16Frontiers in Cellular and Infection MicrobiologyMay 23, 2023Angel K. Kongsomboonvech, Laura García-López, Ferdinand Njume, Felipe Rodriguez, Scott P. Souza, Alex Rosenberg, Kirk D. C. Jensenintegration and double gene deletion. Parasite genomic DNA was isolated using DNAzol (Invitrogen, cat# 10503027) and ethanol precipitation. Diagnostic PCR was performed with MangoMix, which contains MangoTaq DNA polymerase

(4) Late Embryogenesis Abundant Proteins Contribute to the Resistance of Toxoplasma gondii Oocysts against Environmental StressesmBioApril 25, 2023David Arranz-Solís, David Warschkau, Benedikt T. Fabian, Frank Seeber, Jeroen P. J. Saeij(g)DNA was extracted from parasites for screening purposes by using DNAzol reagent (Invitrogen, ref. 10503027). All PCRs were conducted by using either Q5 Hot Start High-Fidelity DNA polymerase (New England Biolabs,

(5) Replication-associated formation and repair of human topoisomerase IIIα cleavage complexesNature communicationsApril 6, 2023Saha LK, Saha S, Yang X, Huang SN, Sun Y, Jo U, Pommier Y.cells (1 × 106) were washed with PBS and lysed by adding 1 mL DNAzol (ThermoFisher Scientific, CAT#:10503027). Nucleic acids were precipitated following addition of 0.5 mL of 100% ethanol, incubation at −20 °C

(6) DUX4 double whammy: The transcription factor that causes a rare muscular dystrophy also kills the precursors of the human noseScience AdvancesFebruary 17, 2023Kaoru Inoue, Hamed Bostan, MaKenna R. Browne, Owen F. Bevis, Carl D. Bortner, Steven A. Moore, Aaron A. Stence, Negin P. Martin, Shih-Heng Chen, Adam B. Burkholder, et al.Each clone was examined for genomic DNA sequences after preparing genomic DNA with DNAzol (Invitrogen, 10503027) or DNeasy (Qiagen, 69504) and performing PCR using primer set 735/736 and/or Western blotting with

(7) Defective DNA polymerase beta invoke a cytosolic DNA mediated inflammatory responseFrontiers in ImmunologyDecember 23, 2022Shengyuan Zhao, Julia A. Goewey Ruiz, Manu Sebastian, Dawit Kidanepreviously (45). Abasic site quantification Genomic DNA was extracted using DNAzol® Reagent (Cat. 10503027, Thermo Fisher) to minimize base loss during sample preparations. DNA was diluted in TE buffer to reach

(8) NEDDylated Cullin 3 mediates the adaptive response to topoisomerase 1 inhibitorsScience advancesDecember 9, 2022Meroni A, Grosser J, Agashe S, Ramakrishnan N, Jackson J, Verma P, Baranello L, Vindigni A.experimental scheme. After CPT treatment, the medium was removed, and cells were lysed with 1 ml of DNAzol (10503027, Thermo Fisher Scientific) and collected by scraping. Genomic DNA and DNA-protein covalent complexes

(9) Knockout of integrin β1 in induced pluripotent stem cells accelerates skin-wound healing by promoting cell migration in extracellular matrixStem Cell Research & TherapyDecember 1, 2022Yansong Ren, Jinbo Liu, Huijun Xu, Shun Wang, Shirui Li, Meng Xiang, Sifeng Chenfor counting iPSC quantification. We isolated genomic DNA from wound tissues, using DNAzol reagent (#10503027; Thermo Fisher Scientific) according to the manufacturer’s instructions. Each sample was subjected to

(10) High-efficiency quantitative control of mitochondrial transfer based on droplet microfluidics and its application on muscle regenerationScience AdvancesAugust 19, 2022Jiayu Sun, Hiu Tung Jessica Lo, Lei Fan, Tsz Lam Yiu, Adnan Shakoor, Gang Li, Wayne Y. W. Lee, Dong Suncontrol. Mitochondrial DNA content Total DNA was extracted from cells using DNAzol (Thermo Scientific, 10503027). The concentrations of total DNA were measured using a NanoDrop 1000 spectrophotometer (Thermo Scientific).

(11) A RADAR method to measure DNA topoisomerase covalent complexesMethods in EnzymologyAugust 7, 2022Alice Meroni, Alessandro Vindigni"DNAzol™ Reagent, for isolation of genomic DNA from solid and liquid samples",Thermo Fisher Scientific,Cat# 10503027 Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines Human:

(12) Replication Kinetics for a Reporter Merkel Cell PolyomavirusVirusesFebruary 25, 2022Abere B, Zhou H, Shuda M, Stolz DB, Rapchak K, Moore PS, Chang Y.collected for protein and DNA extraction. Total genomic DNA was isolated using DNAzol reagent (cat. no. 10503027; Thermo Fisher, Warrington, UK), according to the manufacturer’s instructions, and resuspended in 0.1×

(13) Construction of a human hTERT RPE-1 cell line with inducible Cre for editing of endogenous genesBiology OpenFebruary 15, 2022Naushin L. Hindul et al.chromosome structure of KRAS gene locus. Genomes were isolated by DNAzol reagent (Thermo Fisher Scientific, #10503027) following the manufacturer's instruction. 10 µg of sample genome DNA was digested by EcoRI, run on

(14) Multi-insecticide resistant malaria vectors in the field remain susceptible to malathion, despite the presence of Ace1 point mutationsPLOS GeneticsFebruary 10, 2022Nadja C. Wipf et al.(Molecular Research Center, Inc., purchased from Thermo Fisher Scientific, Waltham, MA, USA, Cat. No. 10503027) using a battery-powered tissue grinder (Sigma-Aldrich, Cat. No. Z359971) and a plastic pestle in a

(15) Liuwei Dihuang Pills Inhibit Podocyte Injury and Alleviate IgA Nephropathy by Directly Altering Mesangial Cell-Derived Exosome Function and SecretionFrontiers in pharmacologyJanuary 1, 2022Zhu X, Shen X, Lin B, Fang J, Jin J, He Q.for three consecutive days. At week 8, the mice were administered staphylococcal enterotoxin (SEB, 10503027, Thermo Fisher Scientific, Waltham, MA, United States) via tail vein injection once weekly for 3 weeks

(16) Genome-wide analysis of 8-oxo-7,8-dihydro-2'-deoxyguanosine at single-nucleotide resolution unveils reduced occurrence of oxidative damage at G-quadruplex sitesNucleic Acids ResearchDecember 2, 2021Jiao An, Mengdie Yin, Jiayong Yin, Sizhong Wu, Christopher P Selby, Yanyan Yang, Aziz Sancar, Guo-Liang Xu, Maoxiang Qian, Jinchuan Hu(ThermoFisher Scientific, 25300054). Genomic DNA was extracted by 4ml DNAzol (ThermoFisher Scientific, 10503027) with the addition of DTT to a final concentration of 10 mM. DNA pellets were resolved in 300 μl of

(17) Rhizospheric bacteria as potential biocontrol agents against Fusarium wilt and crown and root rot diseases in tomatoSaudi Journal of Biological SciencesDecember 1, 2021Md. Masudur Rahman Khalil, Rosario Alicia Fierro-Coronado, Ofelda PeÃ±uelas-Rubio, Alma Guadalupe Villa-Lerma, Rigoberto Plascencia-Jatomea, RubÃ©n FÃ©lix-GastÃ©lum, Ignacio Eduardo Maldonado-Mendozain LB agar medium for 24 h at 30 °C. Total genomic DNA was collected in DNAzol (Invitrogen, Cat. No. 10503–027, USA) according to the manufacturer. Next, the genomic DNA was used for polymerase chain reaction (PCR).

(18) Plasmodium vinckei genomes provide insights into the pan-genome and evolution of rodent malaria parasitesBMC BiologyDecember 1, 2021Abhinay Ramaprasad, Severina Klaus, Olga Douvropoulou, Richard Culleton, Arnab PainGenomic DNA extraction from the parasite pellet was performed using DNAzol reagent (Invitrogen CAT # 10503027) as per the manufacturer’s instructions. Single-molecule sequencing was performed for five P. vinckei

(19) Meiotic recombination mirrors patterns of germline replication in mice and humansCellAugust 5, 2021Florencia Pratto, Kevin Brick, Gang Cheng, Kwan-Wood Gabriel Lam, Jeffrey M. Cloutier, Daisy Dahiya, Stephen R. Wellard, Philip W. Jordan, R. Daniel Camerini-Oteroassays,Critical commercial assays,Critical commercial assays ,, DNAzol,Thermo Fisher Scientific,Cat# 10503027 Qiaquick PCR purification kit,QIAGEN,Cat# 28104 MinElute PCR purification kit,QIAGEN,Cat# 28004 Chromaspin

(20) Effect of Food Colorants Supplementation on Reactive Oxygen Species, Antioxidant Vitamins Level and DNA DamageSains MalaysianaMay 31, 2021Sadaf Shakoor, Amin Ismail, Zia-Ur-Rahman Zia-Ur-Rahman, Mohd Redzwan Sabran, Norhafizah Mohtarrudinsample of tartrazine and curcumin control and treated groups by using the extraction kit (Catalog #:10503027, DNAzol™ Reagent; Thermo Fisher Scientific, USA ). DNA damage was quantified by quantification colorimetric

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DNAzol Reagent, for isolation of genomic DNA from solid and liquid samples from Thermo Fisher Scientific

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DNAzol Reagent, for isolation of genomic DNA from solid and liquid samples from Thermo Fisher Scientific

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